Rapid'E. coli 2 Agar Desidratado

RAPID’E.coli 2 agar is a selective chromogenic medium used for direct enumeration, without confirmation of colonies, of Escherichia coli and other coliforms in food products and environmental samples (depth method).

Intended Use

RAPID’E.coli 2 agar is a selective chromogenic medium used for direct enumeration, without confirmation of colonies, of Escherichia coli and other coliforms in food products and environmental samples (depth method). 

High Specificity

Based on simultaneous detection of two enzymatic activities:

• β-D-Glucuronidase (β GLUC)
• β-D-Galactosidase (β GAL)

E.coli (GAL+/GLUC+) forms violet to pink colonies and other coliforms (GAL+/GLUC-) form blue to green colonies. Detection of GLUC confers a high specificity to the culture medium. Escherichia coli is one of the only species of Enterobacteriaceae to possess this enzyme.

Most interfering flora are inhibited, especially at 44°C, giving the media high specificity.

Two Easy Protocols

• A single layer and a single plate per dilution for enumeration of E.coli at 44°C
• Enumeration of E.coli and coliforms at 37°C with a single layer on the same plate.

• Simultaneous enumeration of E.coli and coliforms at 37°C without further confirmation
• Enumeration of E.coli at 44°C without further confirmation
• Complete results in 18–24 hs
• Gas production not necessary for differentiation of colonies

The RAPID'E.coli 2 method is certified by the AFNOR certification according to the ISO 16140 requirements (No BRD 07/1-07/93 and NoBRD 07/7-12/04) and AOAC-RI validated (No050601).

Nordval Validation Ref No020.