Rapid'E. coli 2 Agar Pronto para uso

RAPID’E.coli 2 agar is a selective chromogenic medium used for direct enumeration, without confirmation of colonies, of Escherichia coli and other coliforms in food products and environmental samples (depth method).

Intended Use: RAPID’E.coli 2 agar is a selective chromogenic medium used for direct enumeration, without confirmation of colonies, of Escherichia coli and other coliforms in food products and environmental samples (depth method). 

High Specificity: Based on simultaneous detection of two enzymatic activities:

• β-D-Glucuronidase (β GLUC)
• β-D-Galactosidase (β GAL)

E.coli (GAL+/GLUC+) forms violet to pink colonies and other coliforms (GAL+/GLUC-) form blue to green colonies. Detection of GLUC confers a high specificity to the culture medium. Escherichia coli is one of the only species of Enterobacteriaceae to possess this enzyme.

Most interfering flora are inhibited, especially at 44°C, giving the media high specificity.

Two Easy Protocols

• A single layer and a single plate per dilution for enumeration of E.coli at 44°C
• Enumeration of E.coli and coliforms at 37°C with a single layer on the same plate.

• Simultaneous enumeration of E.coli and coliforms at 37°C without further confirmation
• Enumeration of E.coli at 44°C without further confirmation
• Complete results in 18–24 hs
• Gas production not necessary for differentiation of colonies

The RAPID'E.coli 2 method is certified by the AFNOR certification according to the ISO 16140 requirements (No BRD 07/1-07/93 and NoBRD 07/7-12/04) and AOAC-RI validated (No050601).

Nordval Validation Ref No020.