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Rapid'L. mono
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Rapid'L. mono

24 hr results after 24 hr enrichment — simultaneous response for Listeria monocytogenes and Listeria spp.
( Bio-Rad )
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Intended Use: RAPID’L.mono agar is a selective chromogenic medium for direct detection and enumeration of Listeria monocytogenes and Listeria spp. in food and environmental samples: 2 answers on one plate.

A Multipurpose Agar:

RAPID’L.mono agar is the only chromogenic method to be validated for Listeria monocytogenes and Listeria spp. detection on the same plate. RAPID’L. mono agar can also be used for enumeration or confirmation of Listeria monocytogenes.

  • Listeria monocytogenes detection in 24 hr after enrichment *
  • Listeria spp. detection in 24 and 48 hr after enrichment
  • Listeria monocytogenes enumeration in 24 hr without confirmation **
  • Listeria monocytogenes confirmation in 24 hr after iQ-Check™ L.mono II

Rapid and Economical Method: With the RAPID’L.mono detection protocol, full results can be obtained within 48 hr using one plate and one broth.

 



Reading and differentiation of Listeria monocytogenes are very easy due to the unique chromogenic principle (PIPLC activity) of RAPID’L.mono agar and the fermentation of a sugar (xylose).

The blue colonies are Listeria monocytogenes (PIPLC+/xylose–).

The blue colonies with a yellow halo are Listeria ivanovii (PIPLC+/xylose+).

The white colonies with or without yellow halo are other Listeria spp. (PIPLC-/xylose+ or –).

RAPID’L.mono agar is very specific; other bacteria and yeasts are inhibited.

Easy and economical confirmation is also validated in the RAPID’L.mono protocol by spot on AL Agar or by the Rhamnose test in 6 hr.

Other Protocols

 

  • RAPID’L.mono chromogenic plating agar is also of interest as a second selective medium in different standardized protocols (for example, ISO 11290 reference method)
  • To confirm positive iQ-Check Listeria monocytogenes II tests
  • To enumerate Listeria monocytogenes in 24 hr without confirmation
  • Listeria monocytogenes detection in 24 hr after enrichment *
  • Listeria spp. detection in 24 and 48 hr after enrichment
  • Listeria monocytogenes enumeration in 24 hr without confirmation **
  • Listeria monocytogenes confirmation in 24 hr after iQ-Check™ L.mono II

 

Other Protocols

  • RAPID’L.mono chromogenic plating agar is also of interest as a second selective medium in different standardized protocols (for example, ISO 11290 reference method)
  • To confirm positive iQ-Check Listeria monocytogenes II tests
  • To enumerate Listeria monocytogenes in 24 hr without confirmation
Validations



*Within the scope of ISO 16140 NordVal validation, no confirmation is required

**Within the scope of the AFNOR validation, confirmation is not required when the presence of Listeria monocytogenes has been confirmed at the detection stage.
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