PDFPrintE-mail


Pathfinder Rotavirus
Ampliar imagem

Pathfinder Rotavirus

The Bio-Rad Pathfinder™ Rotavirus kit is a qualitative enzyme immunoassay for the direct detection of human rotavirus in stool specimens.
( Bio-Rad )
Alguma questão sobre este produto? Clique aqui!

The Bio-Rad Pathfinder™ Rotavirus kit is a qualitative enzyme immunoassay for the direct detection of human rotavirus in stool specimens. Results are available in just 75 minutes for single or batch testing. Pathfinder Rotavirus is a rapid and simple test for rotavirus infection, which can significantly aid in patient management. It uses polyclonal and monoclonal antibodies to detect rotavirus directly from patient specimens. This assay combines the specificity and reproducibility of monoclonal antibodies with the speed and efficiency of EIA.

Pathfinder Rotavirus is easy to use, requiring minimal hands-on time and room-temperature incubation. Results can be read visually or by spectrophotometer. Polyclonal antibody-coated tubes provide efficiency of capture, resulting in high sensitivity of 97 percent. Monoclonal antibodies in the conjugate provide specificity in the detection of captured antigen, resulting in a specificity of 98 percent for complete confidence in results.

Herpes Simplex Virus 1/2 DFA

Pathfinder® HSV Identification in Direct Clinical Specimen Results by Investigator

TP FP FN TN Inadequate Total No. (%) Total
Pathfinder Reagents + + – –
Cell Culture Method + – + –
Investigator A 34 0 6 18 11 (16%) 69
Investigator B 21 1 6 18 3 (6%) 49
In-house 26 0 8 7 6 (13%) 47
Total 81 1 20 43 20 165
Sensitivity = TP/(TP + FN) = 80% Specificity = TN/(TN + FP) = 98%

HSV Identification in Direct Clinical Specimen Results by Sex and Site of Lesion

Predictive Value
Site of Lesion Male Female Sensitivity
TP/(TP + FN)		 Specificity
TN/(TN + FP)		 Positive
TP/(TP + FP)		 Negative
TN/(TN + FN)
Genital 69 79%
38/48		 95%
20/21		 97%
38/39		 67%
20/30
Genital 43 81%
25/31		 100%
12/12		 100%
25/25		 67%
12/18
Anal 8 3 71%
5/7		 100%
4/4		 100%
5/5		 67%
4/6
Other 10 11 86%
12/14		 100%
7/7		 100%
12/12		 78%
7/9
Unknown 1
Total 88 57


Note: The calculations in the two tables are based on comparison to cell culture as the reference method. TP = true positive, FP = false positive, FN = false negative, TN = true negative.

Herpes Simplex Virus 1 and 2 IgG EIA Kit

Interpretation of Bio-Rad HSV 1 and 2 IgG Results

Index Interpretation
< 0.9 Negative for anti-HSV; presumed no previous exposure
≥ 0.9 <1.1 Equivocal
≥ 1.1 Positive for anti-HSV; presumed exposure to HSV type 1 or type 2

Bio-Rad HSV 1 and 2 IgG

Comparative Assay Positive Equivocal Negative 95% Cl
Type 1 HSV
Positive 158 (36) 0 0 Relative Sensitivity 97.1–100 **
Negative 11 (2) 8 30 (2) Relative Specificity* 59.6–86.7 ***
Overall Agreement* 91.3–97.6 ***
Type 2 HSV
Positive 153 (35) 0 0 Relative Sensitivity 97.6–100 **
Negative 16 (3) 8 30 (2) Relative Specificity* 51.5–79.0 ***
Overall Agreement* 88.2–95.7 ***


* Excluding equivocal results.
** Calculated by the Exact Method.
*** Calculated by the Normal Method
( ) Female donors of childbearing age.

Results of tests of 207 specimens (100% frozen). Forty (19%) of the specimens tested were obtained from women of childbearing age.



Alguma questão sobre este produto? Clique aqui!




Produtos vistos recentemente

more categories


Cepas Padrão

Cepas Padrão

Micologia

Micologia

Bacteriologia

Virologia